University of Minnesota, Morris

Link from web directory: yes

Open to U. Relations: yes

Updated: 20 August 2009

CURRICULUM VITAE

Name: Timna J.O. Wyckoff

Title: Associate Professor of Biology

Unit: Division of Science and Mathematics

Campus Address: 1370 Science Building Home Address: 1111 Park Avenue

Morris, MN 56267

Campus Phone: (320) 589-6352 Home Phone: (320) 589-0402

EDUCATION

College and University Education

Ph.D., Biochemistry - Duke University, Durham, NC - October 1998

advisor: Dr. Christian R.H. Raetz

thesis title: Active Site and Acyl Chain Length Specificity of UDP-N-Acetylglucosamine 3-O-Acyltransferases

B.A., Chemistry - University of Minnesota, Morris, Morris, MN - June 1994

with honors, high distinction

Undergraduate Honors and Awards

1994 Curtis A. Larson Award (U of MN, Morris student graduation speaker)

1994 Middle West (District V) Rhodes Scholarship Finalist

1993-94 Elected student rep. to U of MN, Morris Chemistry Department

1993-94 Barry M. Goldwater Excellence in Scholarship Award

Graduate Honors and Awards

1994-98 James B. Duke Fellowship, Duke University Graduate School

1994-97 National Science Foundation Pre-Doctoral Fellowship

EMPLOYMENT

Professional Employment

Associate Professor of Biology, University of Minnesota, Morris, Morris, MN – August 2008-present

Assistant Professor of Biology, University of Minnesota, Morris, Morris, MN – August 2001- August 2008

Visiting Professor, Nurse Anesthesia Program, Wake Forest University Baptist Medical Center, Winston-Salem, NC – Fall 2000

Research Fellow, Wake Forest University School of Medicine, Winston-Salem, NC - August 1999-August 2000

Research Fellow, Duke University Medical Center, Durham, NC - October 1998- July 1999

Sabbaticals and Leaves of Absence

Single-semester Leave – Fall 2005

Maternity Leave – Spring/Summer 2003

Maternity Leave – 2000-2001 academic year

PROFESSIONAL MEMBERSHIPS

Professional Organization Memberships

American Society for Microbiology (ASM), since 2003

American Association for the Advancement of Science (AAAS), since 2001

TEACHING AND ADVISING

University of Minnesota, Morris Courses Taught

*IS1036: The Reemerging Threat of Infectious Disease (Summer Scholars – co-taught with P. Wyckoff) – Summer 2005, 2006, 2007, 2008

IS4994H: Senior Honors Project – Fall 2004 (1 student), Spring 2005 (1 student)

*Biol4901: Senior Seminar - Fall 2001 (1 student), Fall 2002 (1 student), Spring 2003 (1 student), Fall 2003 (1 student), Spring 2004 (2 students), Fall 2004 (1 student), Spring 2005 (1 student), Fall 2005 (1 student), Spring 2006 (1 student), Fall 2006 (1 student), Fall 2007 (1 student), Fall 2008 (1 student), Spring 2009 (1 student)

Biol3701: Biological Communications - Spring 2002 (1 student), Fall 2002 (1 student), Fall 2003 (3 students), Spring 2004 (3 students), Fall 2004 (3 students), Spring 2005 (4 students), Spring 2006 (2 students), Fall 2006 (3 students), Spring 2007 (2 students)

*Biol3701: Biological Communications II (course name change due to reorganization of curriculum): Fall 2007 (1 student), Spring 2008 (3 students), Spring 2009 (3 students)

*Biol4211: Biochemistry - Fall 2001, 2002, 2003, 2004, 2006, 2007, 2008, 2009

*Biol4611: Biochemistry Lab - Fall 2001, 2002, 2003, 2004, 2006, 2007, 2008, 2009

*Biol4111: Microbiology (includes lab) - Spring 2002, 2003, 2004, 2005, 2006, 2007, 2008, 2009

Advising and Instruction-Related University Activities

*36 active advisees as of August 2009

Student research projects supervised:

*Summer 2009: Jackie Hanson (STEP), Tracie Weber( STEP)

*Spring 2009: KauChee Vang (UROP), Jim Davison (UROP), Josie Skala (MMP)

*Fall 2008: KauChee Vang (UROP), Jim Davison (UROP), Josie Skala (MMP)

*Summer 2008: Josie Skala (STEP)

Spring 2008: Meredith Lorig (UROP), KauChee Vang (MMP)

Fall 2007: Laura Nygaard (UROP), Meredith Lorig (UROP), KauChee Vang (MMP)

Spring 2007: Benjamin Buer (UROP), Justin Greiman (UROP)

Fall 2006: Tyler Helland, Benjamin Buer (UROP), Justin Greiman (UROP)

Spring 2006: Becky Bombyk, Tyler Helland

Fall 2005: Becky Bombyk, Brent Hansen (UROP, co-advisor)

Spring 2005: Carolyn Draper (UROP), Elizabeth Savelkoul (UROP)

Fall 2004: Adria Bykowski (UROP), Laura Sullivan (UROP)

Spring 2004: Jenny Montgomery (UROP), Meredith Noetzel

Fall 2003: Jenny Montgomery, Meredith Noetzel

Summer, Fall 2002: Alisha Block (UROP), Kristen Kalina (UROP)

Educational Development Program Grant, “Updating Biol 4611: Biochemistry Laboratory”, Summer 2006; awarded $689 salary, $500 supplies

Faculty Center for Learning and Teaching, UMM Faculty Enrichment Project, 2002-03 (with J. Anderson)

Faculty Center for Learning and Teaching, Teaching Partnership Program, Spring 2002 (with T. Turner)

Faculty Center for Learning and Teaching, “Tenure-tracking” seminar, 2001-02

SCHOLARLY, PROFESSIONAL AND CREATIVE ACTIVITY

Publications

Articles(UMM student co-authors are underlined):

*Bombyk, R.A.M., Bykowski, A.L., Draper, C.E., Savelkoul, E.J., Sullivan, L.R. and Wyckoff, T.J.O. (2008) Comparison of types and antimicrobial susceptibility of Staphylococcus from conventional and organic dairies in west-central Minnesota, USA. J Appl Microbiol. 104(6), 1726-31.

Jain, N.U., Wyckoff, T.J.O., Raetz, C.R.H., Prestegard, J.H. (2004) Rapid Analysis of Large Protein-protein Complexes using NMR-derived Orientational Constraints: The 95 KDa complex of LpxA with Acyl Carrier Protein. J. Mol. Biol. 343(5), 1379-89.

Wozniak, D.J., Wyckoff, T.J.O., Starkey, M., O’Toole, G.A. and Parsek, M.R. (2003) Alginate is not a significant component of the extracellular polysaccharide matrix of PA14 and PAO1 Pseudomonas aeruginosa biofilms. Proc. Nat’l. Acad. Sci. 100(13), 7907-7912.

Wyckoff, T.J.O., Thomas, B., Hassett, D.J. and Wozniak, D.J. (2002) Static growth of mucoid Pseudomonas aeruginosa selects for non-mucoid variants that have acquired flagellum-dependent motility. Microbiol. 148 (11), 3423-3430.

Wyckoff, T.J.O., and Wozniak, D.J. (2001) Transcriptional analysis of genes involved in Pseudomonas aeruginosa biofilms. Methods Enzymol. 336, 144-151.

Wyckoff, T.J.O., and Raetz, C.R.H. (1999) The active site of Escherichia coli UDP-N-acetylglucosamine O-acyltransferase: Chemical modification and site-directed mutagenesis. J. Biol. Chem. 274 (38), 27047-27055.

Wyckoff, T.J.O., Lin, S., Cotter, R.J., Dotson, G.D., and Raetz, C.R.H. (1998) Hydrocarbon rulers in UDP-N-acetylglucosamine Acyltransferases. J. Biol. Chem. 273 (49), 32369-32372.

Odegaard, T.J., Kaltashov, I.A., Cotter, R.J., Steeghs, L., van der Ley, P., Khan, S., Maskell, D.J., and Raetz, C.R.H. (1997) Shortened hydroxyacyl chains on lipid A of Escherichia coli cells expressing a foreign UDP-N-acetylglucosamine O-acyltransferase. J. Biol. Chem. 272 (32), 19688-19696.

Reviews:

Wyckoff, T.J.O., Raetz, C.R.H., and Jackman, J.E. (1998) Antibacterial and anti-inflammatory agents that target endotoxin. Trends in Microbiology. 6 (4), 154-159.

Conference Papers and Presentations (UMM student co-authors are underlined):

*Skala, J.R., Vang, K., Nygaard, L.A. and Wyckoff, T.J.O. (2009) Examination of Phenotypic and Genotypic Pirlimycin Resistance in Staphylococcus from Milk Samples from Conventional and Organic Dairies in West-central Minnesota. American Society for Microbiology 109^th General Meeting; Philadelphia, PA.

*Skala, J.R., Vang, K. and Wyckoff, T.J.O. (2008) Distribution of lincosamide inactivation gene lnu(A) in Staphylococcus strains phenotypically sensitive to the lincosamide pirlimycin. North Central Branch of the American Society for Microbiology 68^th Annual Meeting, St. Cloud, MN.

Bombyk, R.A.M., Helland, T.J. and Wyckoff, T.J.O. (2007) Characterization of tetracycline resistance determinants in Staphylococcus from conventional and organic dairy cows in west-central Minnesota. American Society for Microbiology 107^th Annual General Meeting; Toronto, ON, CANADA.

Helland, T.J. and Wyckoff, T.J.O. (2006) Tetracycline resistance determinants in Staphylococcus isolated from milk samples from conventional and organic dairies in west-central Minnesota. North Central Branch of the American Society for Microbiology 66^th Annual Meeting, Brookings, SD. (received first place in undergraduate poster competition)

Bombyk, R.A.M. and Wyckoff, T.J.O. (2006) Comparison of prevalence and antibiotic resistance of Staphylococcus in milk samples from conventional and organic dairies in west-central Minneosota. American Society for Microbiology 106^th Annual General Meeting, Orlando, FL.

Draper, C.E. and Wyckoff, T.J.O. (2005) Frequency and antibiotic resistance of Staphylococcus in milk samples from conventional and organic dairies in west central Minnesota. North Central Branch of the American Society for Microbiology 65^th Annual Meeting, Ames, IA.

Wyckoff, T.J.O. and Raetz, C.R.H. (1998) Chain length specificity and mechanism of UDP-GlcNAc O-acyltransferase. The Fifth Conference of the International Endotoxin Society, Santa Fe, NM. (oral presentation)

Wyckoff, T.J.O., Lin, S., Cotter, R.J., and Raetz, C.R.H. (1998) Altering the acyl chain specificity of UDP-GlcNAc O-acyltransferases from E. coli and P. aeruginosa. FASEB J. 12 (8), A1286, Abst #L53. Molecular Characterization of Membrane Lipid Metabolism Satellite Meeting to the Annual Meeting of the American Society for Biochemistry and Molecular Biology; Washington, D.C.

Odegaard, T.J. and Raetz, C.R.H. (1996) Probing the active site of UDP-N-acetylglucosamine O-acyltransferase: a trimeric enzyme with a left-handed parallel b helix in its crystal structure. FASEB J. 10 (6), A1236, Abst #1370. Annual Meeting of the American Society for Biochemistry and Molecular Biology; New Orleans, LA.

Grants Received

Within the University:

*Faculty Research Enhancement Fund: Surveillance of antibiotic susceptibility of Staphylococcus in milk samples from a dairy herd while transitioning to organic production. September 19, 2008 (1 year). $1500.

Grant-in-Aid of Research, Artistry and Scholarship: Comparison of bacterial antibiotic resistance on conventional and organic dairies. January 1, 2005 through June 30, 2006 (extended to December 31, 2006 due to illness). $17,854.

Grant-in-Aid of Research, Artistry and Scholarship: Lipid A biosynthesis in Pseudomonas aeruginosa. July 1, 2002 through January 15, 2004 (extended to September 30, 2004 due to maternity leave). $16,361.

Grant Proposals Submitted, Not Accepted

Office of Public Engagement: Determining the effect of dairy antibiotic use and pasture access on the development of bacterial antibiotic resistance. Spring-Fall 2006: $4368.

Council on Undergraduate Research Summer Fellowship: Lipid A biosynthesis in Pseudomonas aeruginosa. Summer 2002.

Research and Scholarly Activities in Progress

The goal of my current research program is to explore agricultural contributions to the development of bacterial antibiotic resistance. Bacterial pathogens that are resistant to currently used antibiotics are emerging as a leading healthcare concern of the 21^st century. Antibiotic resistance is an unavoidable result of antibiotic use. Since over half of the antibiotics produced each year in the U.S. are used in agricultural settings, this is an important arena to consider when assessing the risks of antibiotic use.

My current research project is focused on a comparison of bacterial antibiotic resistance on conventional and organic dairies. In 2004 and 2005, my students and I collected milk samples from both types of dairies, isolated Staphylococcus bacteria from these milk samples and determined the resistance profiles of each strain. A manuscript detailing this data was published early in 2008 in Journal of Applied Microbiology.

More recently, my students have identified resistance genotypes in the subpopulation of antibiotic resistant Staphylococcus from these farms. Posters detailing that work have been presented at various American Society for Microbiology meetings (both regional and national).

Finally, I recently started a collaboration with Dr. Dennis Johnson, dairy scientist at West Central Research and Outreach Center. He began transitioning half of his research dairy herd to organic management in September 2008. We are working together on surveillance of issues surrounding mammary health, including bacterial antibiotic resistance, during the transition.

SERVICE

UMM Service

Committees:

*Search Committee, biology sabbatical replacement; spring 2009

*Search Committee, biology sabbatical replacement; spring 2008

*Premedical Advising Committee; fall 2007-present

*Faculty Affairs Committee; fall 2006-present (elected representative for the Division of Science and Mathematics); chair, fall 2008-present

Commission on Women liaison to the Morris Area Child Care Center Board of Directors; fall 2005-fall 2007, Secretary/Treasurer; January 2006-December 2007

*National Scholarship Advisory Board; 2005-present

Functions and Awards Committee; 2002-2005

Commencement Committee; 2002-2005

Other:

*Biology discipline coordinator, September 2007- January 2009

Latterell Alumni Lecture co-organizer with M. Kuchenreuther, Fall 2006

Thursday Afternoon Faculty Seminar: presented “Comparison of antibiotic resistance on conventional and organic dairies”, Fall 2005

*Supervisor, student worker with stockroom/safety duties: 2003-04 (Rick Jansen), 2004-05 (Tristan Larson), 2005-06 (Sarah Gannon, MSAF), 2006-07 (Emily Archer, MSAF), 2007-08 (Becky Korth, MSAF), 2008-09 (Becky Korth, MSAF)

*Discipline assessment (with P. Wyckoff): designed and continually analyze the data from a survey given to graduating seniors each spring, Spring 2002-present

Undergraduate Research Symposium (UMM) keynote speaker, Spring 2004

Undergraduate Research Symposium (UMM) poster session judge, Spring 2004

Instructional Technology Fair: N. Carpenter and I gave a presentation about using molecular modeling software in a classroom setting, Spring 2003

Biology senior seminar co-coordinator with P. Wyckoff, 2003-04

Professional Service Activities

Biochemistry (American Chemical Society): reviewed a manuscript, December 2006

Invited lecture at West Central Research and Outreach Center, Morris, MN: “Comparing antibiotic resistance on conventional and organic dairies”, January 2006

North Central Branch of the American Society for Microbiology:

65^th Annual Meeting: foreperson of judge panel for undergraduate poster competition, September 2005

66^th Annual Meeting: member of judge panel for undergraduate oral presentation competition, October 2006

68^th Annual Meeting: member of judge panel for undergraduate poster presentation competition, October 2008

Benjamin Cummings Publishing: reviewed a microbiology textbook proposal, February 2004

USDA-ARS (Soils Lab) microbiologist search committee, Spring 2002

Public Service Activities

*Morris Area Elementary School 5^th grade Science Fair: judge, April 2008

*Morris Area High School 7^th and 9^th grade Science Fair: judge, January 2008, 2009

*Science Museum of Minnesota Summer Teacher Institute on Infectious Disease: lead instructor, St. Paul, MN, 25-29 June 2007 and 28 July – 1 August 2008

*Morris Area Elementary School kindergarten classes: UMM Science Building Field Trip (with S. Boyd, T. Pappenfus and P. Wyckoff), Springs 2007, 2008, 2009

Minnesota Science Teachers Association, Chemistry and Physics Conference: keynote speaker, St. Paul, MN, February 2007

*Minnesota Organic Conference and Trade Show: invited speaker, St. Cloud, MN, January 2007, 2009

*Morris Area Community Education Early Childhood and Family Education (ECFE) “Learning is Fun” preschool classes: UMM Science Building Field Trip (with P. Wyckoff), Springs 2006, 2007, 2008

Café Scientifique: presented “Antibiotic use in agriculture: the good, the bad and the controversial”, Minneapolis, MN, May 2006

Café Scientifique: presented “Antibiotic use in agriculture: the good, the bad and the controversial”, Morris, MN, October 2005

Super Saturday Science: Each year I have presented two activities and helped with overall organization for this day of science fun for elementary and junior high school girls on the UMM campus, Falls 2003-2005